Fig. 5.

MoXYL1A accumulated at the Chloroplasts of barley and tobacco seedlings. a Showed the localization pattern of MoXYL1A-GFP during M. oryzae interaction with barley host during early (24–48-hpi), and late (72–96-hpi) stages of pathogen-host interaction. b The micrograph revealed the accumulation of MoXYL1A-GFP to the chloroplast of leaf epidermal tissues of barley leaves at 72-hpi. Scale bar = 10 µm. c and d The micrograph confirmed the co-expression of (MoXYL1A-GFP) and the chloroplast marker (ChCpn10) in the chloroplas of agro-infiltrated tobacco seedlings. Scale bar = 10 µm. e Showed distortions in the localization pattern of MoXYL1A-^∆ctp-GFP. The MoXYL1A-^∆ctp-GFP signals accumulated at the membrane or extracellular regions of agrobacterium infiltrated tobacco seedlings at 48-hpi. GFP was excited at 488 nm and RFP was excited at 561 nm. Scale bar = 20 µm