TABLE 3.
Effects of the cls::mini-Tn10 mutation on resistance of ileS, alaS, and argS mutants to novobiocin and mecillinam
| Strain | Relevant genotype | Resistance (μg/ml) toa:
|
|
|---|---|---|---|
| Novobiocin | Mecillinam | ||
| C600 | cls+ | 12.5 | 0.05 |
| SY456 | cls::mini-Tn10 | 2.0 | 0.05 |
| SY513 | ileS80 cls::mini-Tn10 | 40 | >10 |
| SY504 | alaS21 cls::mini-Tn10 | 60 | >10 |
| SY505 | argS201 cls::mini-Tn10 | 50 | >10 |
| SY703 | SY508(pSM11) | 7.0 | ND |
a
Amounts of novobiocin were increased from 0 to 10 μg/ml by increments of 0.5 μg/ml per plate for strains SY456 and SY703 and from 0 to 70 μg/ml by increments of 10 μg/ml per plate for the other strains. Other experimental details are as described in Table 2, footnotes a and b. ND, not determined.