Figure 6.

ORP5 over-expression induces an increase of the localization of seipin to MAM–LD contact sites. (A) Representative confocal images showing a single focal plane of HeLa cells expressing YFP-seipin (green), Mito-BFP (blue) and Sec22b (red) or ORP5∆PH (red). The LDs were stained LTox (purple). Arrowhead points seipin enrichment at MAM–mitochondria contact sites and arrows mark seipin enrichment at Mito–MAM–LD contact sites. Scale bar, 10 µm. (B) Representative 3D reconstruction images of the different categories for the classification of the localization. (C) Analysis of the localization of seipin enrichments in HeLa cells expressing seipin alone or in co-expression with Sec22b or ORP5∆PH. Data are shown as % mean ± SEM of cell of n = 56 cells in YFP-seipin (expressed alone), n = 14 cells in YFP-seipin + RFP-Sec22b, and n = 40 cells in YFP-seipin + RFP- ORP5∆PH, (** = P < 0.01; Wilcoxon-Mann-Whitney test).