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. 2022 Aug 12;221(9):e202112107. doi: 10.1083/jcb.202112107

Figure 8.

Figure 8.

ORP5 and seipin biochemically interact in lysates of HeLa cell. (A) Western blot analysis of ORP5A or ORP5B immunoprecipitated (IP) from lysates of HeLa cells co-expressing EGFP or seipin-EGFP with HA-ORP5A or HA-ORP5B, using antibodies against GFP (to detect seipin) or OA1 (to detect ORP5A or ORP5B). (B) Western blot analysis of co-IP products from HeLa cells co-expressing EGFP or seipin-EGFP with ORP5A or ORP5∆ORD using antibodies against GFP (to detect seipin) or OA1 (to detect ORP5A or ORP5∆ORD). (C) Relative quantification of ORP5A or ORP5∆ORD co-immunoprecipitated with seipin-EGFP or EGFP alone. (D) Western blot analysis of co-IP products from HeLa cells co-expressing EGFP or seipin-EGFP with HA-ORP5A or HA-ORP5∆TM, using antibodies against GFP (to detect seipin) or OA1 (to detect ORP5A or ORP5∆TM). (E) Relative quantification of ORP5A or HA-ORP5∆TM co-immunoprecipitated with seipin-EGFP or EGFP alone.