Fig. 9.

Schematic illustration of the ExoTIC device for EV isolation. A Various biofluids can be processed. B Schematic illustration of the ExoTIC device. Intact EVs are enriched and purified at the filter, whereas the free proteins and nucleic acids are washed out. C Downstream analysis of isolated EVs. D Schematic of EV isolation from sample-in to EV-out. Total operation time for 5–10 mL of sample is under 3 h. E Design schematic of the ExoTIC device. F Image of an actual modular ExoTIC device. It is comprised of 5 modules, each module with a different membrane pore size, that connect in series for isolation of EVs. Reproduced with permission [56]. Copyright 2017, American Chemical Society