Table 1.
Test | Sperm cells status | Post-thawing incubation time and concentration of L. meyenii | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ||||||||||||||
0 h | 1 h | 3 h | 24 h | |||||||||||
Control | Control | 1 µg/mL | 10 µg/mL | 100 µg/mL | Control | 1 µg/mL | 10 µg/mL | 100 µg/mL | Control | 1 µg/mL | 10 µg/mL | 100 µg/mL | ||
Motility (%) | Rectilinear | 42 | 50 | 46 | 63 | 57 | 54 | 54 | 55 | 53 | 49 | 60 | 53 | 48 |
Circular | 9 | 9 | 7 | 5 | 8 | 5 | 4 | 4 | 7 | 3 | 5 | 3 | 3 | |
Static | 5 | 2 | 8 | 5 | 5 | 0 | 0 | 5 | 3 | 8 | 11 | 15 | 12 | |
Immotile | 45 | 39 | 45 | 28 | 31 | 42 | 43 | 37 | 38 | 42 | 24 | 30 | 39 | |
HOS (%) | Normal | 44 | 46 | 44 | 59 | 47 | 44 | 52 | 47 | 47 | 44 | 42 | 46 | 41 |
Abnormal | 56 | 54 | 57 | 41 | 53 | 56 | 49 | 54 | 54 | 56 | 58 | 54 | 59 | |
Acrosomal | Damaged - Died | 35 | 19 | 26 | 27 | 27 | 19 | 37 | 21 | 29 | 30 | 37 | 34 | 43 |
Integrity (%) | Intact - Died | 22 | 31 | 26 | 42 | 35 | 33 | 29 | 35 | 44 | 49 | 38 | 26 | 27 |
Damaged - Live | 7 | 2 | 1 | 2 | 3 | 15 | 3 | 2 | 2 | 1 | 1 | 1 | 0 | |
Intact - Live | 37 | 48 | 48 | 30 | 36 | 34 | 32 | 43 | 26 | 21 | 25 | 40 | 30 | |
Vitality (%) | Died | 57 | 50 | 52 | 68 | 62 | 52 | 66 | 56 | 73 | 78 | 75 | 60 | 70 |
Live | 44 | 50 | 49 | 32 | 39 | 49 | 35 | 45 | 28 | 22 | 25 | 41 | 30 | |
Morphology (%) | Normal | 91 | 91 | 92 | 91 | 91 | 88 | 90 | 88 | 87 | 88 | 90 | 87 | 87 |
Abnormal – Head | 3 | 2 | 2 | 3 | 3 | 5 | 3 | 2 | 3 | 3 | 3 | 2 | 4 | |
Abnormal – Neck | 3 | 3 | 3 | 3 | 3 | 4 | 3 | 5 | 6 | 6 | 5 | 4 | 5 | |
Abnormal - Tail | 3 | 5 | 3 | 3 | 3 | 3 | 4 | 4 | 5 | 3 | 3 | 5 | 4 | |
TUNEL (%) | Normal DNA | 95.43 | 95.34 | 95.59 | 94.27 | 95.71 | 93.35 | 95.03 | 93.52 | 93.10 | 90.60 | 92.29 | 91.35 | 91.44 |
Fragmented DNA | 4.57 | 4.66 | 4.41 | 5.73 | 4.29 | 6.65 | 4.97 | 6.48 | 6.90 | 9.40 | 7.71 | 8.65 | 8.56 |
HOS=Hypo-osmotic swelling, TUNEL=DNA fragmentation with terminal deoxynucleotidyl transferase dUTP nick end labeling