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. 2022 Jun 14;15(6):1481–1488. doi: 10.14202/vetworld.2022.1481-1488

Table 1.

Sperm quality evaluation tests of Aberdeen Angus treated with three concentrations of Maca and at three different times. The results are expressed as a percentage.

Test Sperm cells status Post-thawing incubation time and concentration of L. meyenii

0 h 1 h 3 h 24 h
Control Control 1 µg/mL 10 µg/mL 100 µg/mL Control 1 µg/mL 10 µg/mL 100 µg/mL Control 1 µg/mL 10 µg/mL 100 µg/mL
Motility (%) Rectilinear 42 50 46 63 57 54 54 55 53 49 60 53 48
Circular 9 9 7 5 8 5 4 4 7 3 5 3 3
Static 5 2 8 5 5 0 0 5 3 8 11 15 12
Immotile 45 39 45 28 31 42 43 37 38 42 24 30 39
HOS (%) Normal 44 46 44 59 47 44 52 47 47 44 42 46 41
Abnormal 56 54 57 41 53 56 49 54 54 56 58 54 59
Acrosomal Damaged - Died 35 19 26 27 27 19 37 21 29 30 37 34 43
Integrity (%) Intact - Died 22 31 26 42 35 33 29 35 44 49 38 26 27
Damaged - Live 7 2 1 2 3 15 3 2 2 1 1 1 0
Intact - Live 37 48 48 30 36 34 32 43 26 21 25 40 30
Vitality (%) Died 57 50 52 68 62 52 66 56 73 78 75 60 70
Live 44 50 49 32 39 49 35 45 28 22 25 41 30
Morphology (%) Normal 91 91 92 91 91 88 90 88 87 88 90 87 87
Abnormal – Head 3 2 2 3 3 5 3 2 3 3 3 2 4
Abnormal – Neck 3 3 3 3 3 4 3 5 6 6 5 4 5
Abnormal - Tail 3 5 3 3 3 3 4 4 5 3 3 5 4
TUNEL (%) Normal DNA 95.43 95.34 95.59 94.27 95.71 93.35 95.03 93.52 93.10 90.60 92.29 91.35 91.44
Fragmented DNA 4.57 4.66 4.41 5.73 4.29 6.65 4.97 6.48 6.90 9.40 7.71 8.65 8.56

HOS=Hypo-osmotic swelling, TUNEL=DNA fragmentation with terminal deoxynucleotidyl transferase dUTP nick end labeling