Table 2.

Primer set for qRT-PCR (AI and ND: M gene) and RT-PCR (ND: F and HN gene).

Primer	Forward	Reverse	Probe
ND_Matrix	5’- ACAACCAAGTGAGGTGAGTACTTG-3’	5’- GACTCCCTTTCTCTGATTGTCCAT-3’	5’FAM-CGTTTCCAGTCGTTGGATTTAC-TAMRA 3’
AI_Matrix	5’ AGATGAGYCTTCTAACCGAGGTCG 3’	5’- TGCAAANACATCYTCAAGTCTCTG-3’	5’FAM-TCAGGCCCCCTCAAAGCCGA-TAMRA 3’
ND_F_Reg1	5’- AGAGTGTGGATCCCAACCAG -3’	5’- GTGGATACAGACCCTTGAATCTTG-3’	-
ND_F_Reg2	5’- GCAGGGATTGTAGTAACAGGAGAT-3’	5’- CCAAGAGTTGAGTCTGTGAGTCAT-3’	-
ND_F_Reg3	5’- ACTACAGTGTTCGGGCCACA-3’	5’- AGCCTCAGAGTTATCCCGTCTAAT-3’	-
ND_F_Reg4	5’- GTTTGAGCGGCAACACATC-3’	5’- GTTCTACCCGTGTATTGCTCTTTG-3’	-
ND_HN_Reg1	5’- CCCACAACATCCGTTCTACC-3’	5’- CGAAGCACACCAAGTGCTAA-3’	-
ND_HN_Reg2	5’- TTAGCACTTGGTGTGCTTCG-3’	5’- ACCGTGAGAATTCTGCCTTC-3’	-
ND_HN_Reg3	5’- TGAGGACCCAATGCTGACTA-3’	5’- CCCCGAATAGGGTATTGGAT-3’	-

qRT-PCR = Quantitative reverse transcription polymerase chain reaction