Figure S1. Pldo is required for hair and laterals formation.

(A, B) Characterization of CG34401/pldo LOF phenotypes. Adult wings with unmarked pldo clones showing the absence of hairs phenotype. In these cases, different alleles for pldo were used (pldo¹ and pldo^B). Scale bars correspond to 100 μm. (C, D) Adult wings showing the pldo LOF phenotype generated by the expression of RNAi (and RNAi against GFP as a control) in the ptcGal4 expression domain in the wing (note that ptc is expressed between veins 2 and 3 in the wing along the A/P compartment boundary, see schematic of ptc expression in Fig 3A). Scale bars correspond to 50 μm. (E, F) pldo LOF phenotype in adult antenna, generated by ey-Gal4 driven RNAi expression (and control RNAi for comparison). Scale bars correspond to 50 μm. (G, H) Adult thorax showing the absence of cuticular hairs generated by the expression of RNAi (and RNAi against GFP as a control) in the pannierGal4 expression domain in the notum (note that bristles are not affected). The scale bar corresponds to 100 μm. (I, J) Quantification of embryo and larvae mutants for pldo (GFP negative) or control (GFP positive). In each experiment, at least 20 embryo/larvae were considered per genotype, n = 3. Two-way ANOVA plus Sidak’s multiple comparison test was used for statistical analysis, **** indicates P < 0.0001; ** indicates P = 0.0039; and * indicates P = 0.0259. (K) pldo is located on the X chromosome at position X: 18,863,950… X: 18,884,075, and BLAST analysis indicates that pldo is conserved from Caenorhabditis elegans to humans. Protein sequence alignment of main predicted domains in Pldo from different species: Cel: C. elegans, Dros: Drosophila, Drer: Danio rerio, and Hsap: Homo sapiens. Alignment was obtained through the software package Clustal Omega (RRID:001591).