Figure S5. N-terminal region of Pldo is sufficient to induce filopodia formation.
(A) PldoΔC was not capable to rescue the lack of trichome phenotype observed by pldo LOF in adult wings, when co-expressing with the UAS-pldo-IR in the ptcGAL4 expression domain. No change in the phenotype was observed, confirming the pldo LOF mutant defects, as seen in pupal wings (Fig 5D). The scale bar represents 50 μm. (B) Localization of Pldo was evaluated in confocal microscopy images, through the HA-tag staining, in hemocytes, observing no notable differences. These experiments were performed at 18°C, with near wild-type expression levels to prevent overexpression of the transgenes. Scale bar represents 5 μm. (C, D) Localization of Pldo was evaluated in confocal microscopy images, through the HA-tag staining, in pupal wings, before and after hair formation, observing no notable differences. (C, D) These experiments were performed at 18°C, at 64 (C), or 72 h (D) APF, with near wild-type expression levels to prevent overexpression of the transgenes. Note at the bottom the colocalization between Pldo-HA and Scar. The scale bar represents 25 μm.