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. 2022 Aug 9;15:4321–4334. doi: 10.2147/IDR.S377797

Table 1.

Primers Sequences and PCR Cycling Conditions

Genes Oligonucleotides Sequences Amplicon size (bp) (35 Cycles) References
Den. Annealing Ext.
16SrRNA (1133fgal)-23SrRNA (114r) F: TATTCTTTGTTACCARCGG
R: GGTTTCCCCATTCGG		 1032 94°C
30 sec		 55°C
50 sec		 72°C
1 min		 [22]
gtxA
(Cytolytic-hemolytic gene) 		F: CAAACCTAATTCAATCGGATG
R: TGCTTCAATAATTTTCCATTTTC		 1257 94°C
30 sec		 51°C
40 sec		 72°C
1 min		 [1]
fifA
(Flageller gene) 		F: CACCATGGGTGCATTTGCGGATGATCC
R: TATTCGTATGCGATAGTATAGTTC		 538 94°C
30 sec		 55°C
40 sec		 72°C
45 sec		 [27]
gyrB
(The gyrase subunit B gene) 		F: TGTGCGTTTCTGGCCAAGTC
R: CGCTCACCAACTGCAGATTC		 561 94°C
30 sec		 55°C
40 sec		 72°C
45 sec		 [28]
blaROB
(Beta lactam resistance gene) 		F: AATAACCCTTGCCCCAATTC
R: TCGCTTATCAGGTGTGCTTG		 685 94°C
30 sec		 60°C
40 sec		 72°C
45 sec		 [29]
aphA1 (Aminoglycosides resistance gene) F: TTATGCCTCTTCCGACCATC
R: GAGAAAACTCACCGAGGCAG		 489 94°C
30 sec		 54°C
40 sec		 72°C
40 sec
tetH
(Tetracycline resistance gene) 		F: ATACTGCTGATCACCGT
R: TCCCAATAAGCGACGCT		 1076 94°C
30 sec		 60°C
40 sec		 72°C
1 min
tetB
(Tetracycline resistance gene) 		F: CCT TAT CATGCC AGT CTTGC
R: ACT GCCGTT TTT TTCGCC		 774 94°C
30 sec		 50°C
40 sec		 72°C
1 min		 [30]