FIG. 9.

Analysis of otrA transcription and mycelial growth at the onset of OTC production by S. rimosus 4018 grown in LCM. (A) Open circles indicate the amount of OTC in samples taken from six cultures (50 ml) that were inoculated at the same time and incubated at 28°C on the same rotary platform. Closed circles indicate the concentration of OTC in cultures used to analyze otc transcripts. Squares indicate the mean dry weight of the mycelium at different stages in the fermentation. The determination of dry weight had a variance of less than 5% error for the mean. (B) S1 nuclease protection analysis of otrA transcription. Assay conditions were as described in the legend to Fig. 8. Lane 1, RNA from 4018 grown in TSB; lanes 2, 3, and 4, RNA from 4018 grown in LCM for 24, 32, and 56 h, respectively.