FIG. 4.

Levels of MHY1 mRNA during the dimorphic transition (A) and under stress conditions (B). Total RNA was isolated from E122 cells that were incubated at 28°C in YNBGlcNAc (for induction of filamentous growth) or YNBGlc (for control culture, yeast-like cells) for the times indicated (A) or that were exposed to several stress conditions (B). For carbon source starvation, cultures were sampled at 0, 1, and 5 h (lanes 1, 2, and 3, respectively). For heat shock and osmotic and oxidative stresses, cultures were sampled at 0, 30, and 120 min (lanes 1, 2, and 3, respectively). RNA (10 μg) from each time point was separated on a formaldehyde agarose gel, transferred to nitrocellulose, and subjected to Northern blot analysis. The blots were hybridized with a probe specific for the MHY1 gene. Equal loading of RNA was ensured by ethidium bromide staining (data not shown). In panel A, cell morphologies at t = 0, 3, and 10 h are shown.