FIG. 5.

Nuclear localization of Mhy1p during filamentous growth of Y. lipolytica. Indirect immunofluorescence of HA-tagged Mhy1p was carried out on yeast-like cells (A) and at different stages of filamentation (early germ tube formation [B], late germ tube formation [C], and hyphal growth [D]) as described in Materials and Methods. Left panels, cells visualized by bright-field microscopy; middle panels, DAPI staining of nuclei; right panels, rhodamine (tetramethyl rhodamine isothiocyanate) staining observed with an anti-HA monoclonal antibody. Bars = 5 μm.