Table 1.
Summary of the results from full-length sequencing of 12 samples
| Sample IDa | Clean Data | Full-length readsc | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Total reads number | Clean Numberb | N50 (bp) | Mean length (bp) | Max length (bp) | Reads number | N50 (bp) | Mean length (bp) | Max length (bp) | Mapped reads (Rate) | |
| BCD1 | 4,298,610 | 4,127,858 | 1,183 | 1,036 | 24,701 | 3,609,340 | 1,034 | 824 | 11,258 | 3,498,849 (96.9%) |
| BCD2 | 4,552,299 | 4,382,518 | 1,126 | 984 | 58,131 | 3,869,358 | 986 | 775 | 9,704 | 3,736,319 (96.6%) |
| BCD3 | 4,812,931 | 4,624,033 | 980 | 886 | 42,651 | 4,101,426 | 828 | 682 | 6,950 | 3,938,757 (96.0%) |
| BCK1 | 6,541,618 | 6,309,821 | 695 | 661 | 25,330 | 5,744,254 | 552 | 460 | 8,480 | 5,421,952 (94.4%) |
| BCK2 | 5,594,616 | 5,389,905 | 732 | 696 | 26,878 | 4,840,205 | 590 | 496 | 9,278 | 4,596,506 (95.0%) |
| BCK3 | 5,800,487 | 5,586,539 | 821 | 750 | 95,008 | 5,030,650 | 678 | 547 | 8,644 | 4,779,199 (95.0%) |
| ICD1 | 4,654,566 | 4,471,028 | 1,002 | 906 | 71,935 | 4,051,667 | 862 | 697 | 11,677 | 3,861,154 (95.3%) |
| ICD2 | 3,890,121 | 3,759,176 | 1,265 | 1089 | 15,250 | 3,297,036 | 1,122 | 881 | 10,402 | 3,173,572 (96.3%) |
| ICD3 | 4,797,794 | 4,620,255 | 1,034 | 932 | 14,970 | 4,085,571 | 891 | 722 | 10,777 | 3,903,260 (95.5%) |
| ICK1 | 6,126,592 | 5,906,096 | 750 | 718 | 233,119 | 5,316,572 | 605 | 513 | 9425 | 4,983,658 (93.7%) |
| ICK2 | 6,119,503 | 5,872,403 | 698 | 680 | 202,515 | 5,331,339 | 550 | 476 | 10,613 | 4,998,266 (93.8%) |
| ICK3 | 5,523,159 | 5,322,332 | 833 | 772 | 85,786 | 4,792,444 | 684 | 567 | 8969 | 4,527,555 (94.5%) |
aSample ID: inbred line (B and I represent B73 and IL85 respectively) + treatment (CK and CD represent control and cold stress respectively) + replication;
bNumber of reads after eliminating rRNA;
cReads with primers at both ends