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. 2022 Aug 10;17:3583–3599. doi: 10.2147/IJN.S362244

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© 2022 Chen et al.

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Characterization of Cucumber-derived nanovesicles (CDNVs). (A and B) TEM images of CsDNVs obtained under a centrifugal force of 100,000 g for 60 min and 30 min, respectively. (C and D) TEM images of CpDNVs obtained under a centrifugal force of 100,000 g for 60 min and 30 min, respectively. Scale bar: 200 nm. (E and F) Diameters of CsDNVs and CpDNVs, respectively, obtained under different centrifugal forces. **p < 0.01 vs 30,000 × g group. ***p < 0.001 vs 30,000 × g group. N=3. (G and H) TEM images of CsDNVs and CpDNVs, respectively, obtained under a centrifugal force of 30,000 g for 30 min. Scale bar: 200 nm. (I and J) Distribution of diameters of CsDNVs and CpDNVs, respectively, obtained under a centrifugal force of 30,000 g for 30 min. (K and L) Zeta potentials of CsDNVs and CpDNVs, respectively, obtained under a centrifugal force of 30,000 g for 30 min. (M), (N and O) LC-MS analysis of CuB, CsDNV and CpDNV content, respectively.