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. Author manuscript; available in PMC: 2022 Aug 14.
Published in final edited form as: Dev Biol. 2021 Jan 19;472:52–66. doi: 10.1016/j.ydbio.2021.01.007

Figure 6-. Photoconverted cardiomyocytes counted manually from a z-stack of the larval heart.

Figure 6-

A- 48 hpf left lateral side 3D stack of the heart, displaying every fourth stack of 2.0 μm. Red fluorescence shows right side lateral aLPM-derived Kaede-expressing cells photoconverted at 18 hpf. B-E Insets of the heart showing examples of how cardiomyocytes were counted with numbered points in yellow. This embryo had a total of 185 red fluorescent cardiomyocytes counted, which was verified by the average of three separate manually counted analyses.