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. 2022 Aug 13;5:814. doi: 10.1038/s42003-022-03785-z

Fig. 2. Comparison of extended HSQC spectra of the H2A and H2B tails between the 112-bp DNA/pAID nucleosome alone (red) and with the two-fold addition of 33-bp DNA (blue).

Fig. 2

a, b NMR signals are divided into panels by amino acid residue of H2A (a) and H2B (b). Signal assignments in the 112-bp DNA/pAID nucleosome alone and with the two-fold addition of DNA at 0 mM NaCl are labeled in red and blue, respectively. Two-fold addition of 33-bp DNA to the 112-bp DNA/pAID nucleosome leads to a double-strand break nucleosome wrapped by 112-bp and 33-bp DNA (112/33-bp nucleosome), as shown in Supplementary Fig. 2a. Filled circles indicate each signal center. The signal centers were identified from peaks in the slice representations of signals in Supplementary Fig. 1. pAID and DNA side signals are designated by subscript p and d, respectively. High- and low-field components of the signals are designated by subscript h and l, respectively. Arrows represent chemical shift changes upon the addition of 33-bp DNA.