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. 2022 Aug 13;8:360. doi: 10.1038/s41420-022-01152-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Differential metabolites were enriched in the glucagon signaling pathway and AMPK signaling pathway using KEGG databases. B Metabolic changes of ATP and AMP were detected by targeted metabolomics analysis. C Schematic diagram of the AMPK signaling pathway as presented in the KEGG database (http://www.genome.jp/kegg/). D Spearman’s correlation analysis of KCNK3 and AMPK subunits in LUAD patients from TCGA by using clinical bioinformatics database. E, F Relative p-AMPK, AMPK, TXNIP protein expression was measured by western blot after transfection with KCNK3 lentivirus in H1975 cells and H1299 cells, with results normalized relative to the expression of GAPDH. (Data are presented as the mean ± SD of three independent experiments. ****P < 0.0001).