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. 2022 Aug 13;8:360. doi: 10.1038/s41420-022-01152-9

Fig. 6. KCNK3 inhibited proliferation and glucose metabolism via activation of the AMPK-TXNIP pathway in LUAD cells.

Fig. 6

A, B The expression of KCNK3, GLUT1, LDHA, p-AMPK, AMPK, TXNIP was detected by western blot in LUAD cells after administration with AMPK inhibitor (Dors, Dorsomorphin), with results normalized relative to the expression of GAPDH. C, D The proliferative curves of LUAD cells were detected by CCK-8 assay and expressed as OD values. E, F Clone formation assays were performed to evaluate the proliferation of KCNK3 overexpression LUAD cells after treating with AMPK inhibitor. G, H Relative glucose uptake and lactate production were measured in KCNK3-overexpressed or control LUAD cells after AMPK inhibitor treatment. (Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ##P < 0.01, ###P < 0.001, ####P < 0.0001).