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. 2022 Aug 13;13(8):703. doi: 10.1038/s41419-022-05157-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A RNA-seq analysis showing that CYTOR expression is associated with 1294 genes dysregulation in Cal27 cells. B Gene set enrichment analysis (GSEA) of the CYTOR-related pathways in CYTOR-knockdown Cal27 cells. C Western blot analysis showing the mitochondrial-related proteins (SIRT3, CTP, SOD1, SOD2) expression in CYTOR-depletion cells. D Flow cytometry analysis of ROS level (MitoSOX) in HN6 and Cal27 cells. E, F CYTOR-knockdown and CYTOR-overexpression HN6 and Cal27 cells were detected for ECAR to indicate the glycolysis stress. Median values are plotted in the column with data generated from six technical replicates and three biological replicates (each dot indicates the mean value of three independent biological replicates), statistical analysis was performed by unpaired t-tests. G, H CYTOR-knockdown and CYTOR-overexpression HN6 and Cal27 cells were detected for OCR to indicate the mitochondrial respiration. I Transmission electron microscope exposing the ultrastructure of mitochondrial in HN6 and Cal27 cells. Median values are plotted in the dots plot with data generated from four independent biological replicates, statistical analysis was performed by unpaired t-tests (KD knockdown, NC negative control; scale bar = 1 μm. The data was presented as mean ± SD, *P < 0.05).