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. 2022 Aug 13;5:816. doi: 10.1038/s42003-022-03788-w

Fig. 7. TDP-43 facilitates lipid metabolism via ABHD2 to suppress apoptosis in vitro.

Fig. 7

a Analysis of FFA levels in SMMC-7721 (left) and MHCC97H (right) cells. The cells were transiently transfected with si-Control/si-TDP-43#2 and pCMV3 (vector)/pCMV3-ABHD2 to silence TDP-43 or overexpress ABHD2. Quantitative analysis (b) and fluorescence images (c) of reactive oxygen species (ROS) in SMMC-7721 and MHCC97H cells after transient transfection with the displayed siRNAs or plasmids. Scale bar: 200 µm. d Flow cytometry analysis of apoptosis in SMMC-7721 (upper panel) and MHCC97H (lower panel) cells upon TDP-43 deletion along with or without ABHD2 overexpression. e Quantitative analysis of apoptotic rate in (d). f qRT-PCR assay for the mRNA levels of TDP-43, ABHD2 and Bcl-2 in SMMC-7721 cells upon TDP-43 deletion along with or without ABHD2 overexpression. The data was normalized to the mRNA level of GAPDH. g Western blot analysis of protein levels of ABHD2, p53, Bcl-2, C-caspase3, Ki67, and TDP-43 in SMMC-7721 and MHCC97H cells. Group number 1, 2, and 3 separately represents the three groups shown in (f). The cells were transiently transfected with the displayed siRNAs or plasmids. The values in the graphs represent the mean of three biologically independent experiments. Error bars represent ± s. d. *P < 0.05, **P < 0.01, ***P < 0.001 by two-tailed Student’s t test.