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. 2022 Aug 13;13:4753. doi: 10.1038/s41467-022-32417-7

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Investigation of three different concentrations of BSA: 20 (red), 40 (blue), and 60 mg ml⁻¹ (violet) and extraction of the transition temperature x0. a Absolute measurement values (circles) and sigmoidal Boltzmann-Fit curves (solid lines) in absorbance units (AU). b Comparison of the (individually normalized) Boltzmann-fit curves, showing the temperature- and concentration-dependence of the sigmoidal-shaped absorbance curves.