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. 2022 Aug 13;12:13797. doi: 10.1038/s41598-022-17489-1

Table 2.

Summary of identified studies using optical imaging as the screening modality.

Study Data source ML classification methods Performance metrics Outcomes (best performing ML)
Optical imaging
Uthoff et al.30 Custom smartphone-based dual modality device capable of both white light and autofluorescence imaging NN based on VGG-M architecture, pre-trained on ImageNet

Sensitivity

Specificity

Precision

NPV

Accuracy

AUROC

Sensitivity 85.0

Specificity 89.0

Precision 0.88

NPV 0.85

Accuracy 86.9

AUROC 0.91

Song et al.17 Smartphone-based intraoral imaging system with custom WL probe NN based on VGG19 architecture, pre-trained on ImageNet Accuracy Accuracy 85.6
Chan et al.31 VELscope device32 Classification based ResNet or Inception architecture, using either a fully convolutional network or feature pyramid network

Sensitivity

Specificity

Sensitivity 98.0

Specificity 88.0

Aubreville et al.33 Confocal Laser Endomicroscopy images of oral cavity following IV fluorescein. Images extracted from IO videos. CystoFlex UHD and Coloflex UHD as imaging devices Used untrained LeNet-5 architecture with patch probability fusion, whole image classification using pre-trained Inception V3 CNN and random forest classifier. Best performance using LeNet-5

Sensitivity

Specificity

Accuracy

AUROC

Sensitivity 86.6

Specificity 90.0

Accuracy 88.3

AUROC 80.7

De Veld et al.15 Xe lamp with monochromator for illumination, a spectrograph and custom set of long-pass and short-pass filters NN with base architecture not specified; single hidden layer between input and output AUROC AUROC 0.68
Roblyer et al.34 Multispectral digital microscope (MDM), measuring white light reflectance, autofluorescence, narrow band reflectance and cross-polarised light Linear discriminant analysis

Sensitivity

Specificity

AUROC

Sensitivity 93.9

Specificity 98.1

AUROC 0.981

Caughlin et al.35 Multispectral autofluorescence lifetime imaging (maFLIM) endoscopy Bespoke neural network using a shared encoder and separate paths for signal reconstruction and classification; classification on pixel-pixel basis

Sensitivity

Specificity

Precision

Accuracy

F1

Sensitivity 87.5

Specificity 67.6

Precision 76.3

Accuracy 77.6

F1 0.80

Jo et al.36 Time-domain multispectral FLIM rigid endoscope. Emission spectral collected for collagen, NADH, FAD Quadratic discriminant analysis

Sensitivity

Specificity

AUROC

Sensitivity 95

Specificity 87

AUROC 0.91

Francisco et al.37 Portable spectrophotometer with two solid state lasers; a diode emitting at 406 nm and a double frequency neodymium 523 nm as excitation source Compared naïve bayes, k-Nearest Neighbours and decision tree. Decision tree provided best performance

Sensitivity

Specificity

Accuracy

Sensitivity 87.0

Specificity 91.2

Accuracy 87.0

Wang et al.19 Fibre optics-based flurospectrometer, using Xe lamp with monochromator as excitation source Partial least squares combined with artificial neural network—neural network with single hidden layer

Sensitivity

Specificity

Precision

Sensitivity 81.0

Specificity 96.0

Precision 88

Majumder et al.38 N2 laser as excitation source Relevance Vector Machine (RVM)

Sensitivity

Specificity

AUROC

Sensitivity 91

Specificity 95

AUROC 0.9

Huang et al.39 VELscope device Quadratic discriminant analysis

Sensitivity

Specificity

Sensitivity 92.3

Specificity 97.9

Duran-Sierra et al.40 Multispectral autofluorescence lifetime imaging endoscopy (maFLIM); preferential excitation of NADH and FAD Best performance using ensemble approach of support vector machine and quadratic discriminant analysis

Sensitivity

Specificity

F1

AUROC

Sensitivity 94.0

Specificity 74.0

F1 0.85

AUROC 0.81

Jeng et al.41 VELscope device Used both linear discriminant analysis and quadratic discriminant analysis

Sensitivity

Precision

Accuracy

F1

AUROC

Sensitivity 92.0

Precision 0.86

Accuracy 86.0

F1 0.88

AUROC 0.96

Huang et al.42 Custom autofluorescence device, comprising two LED continuous wave lamps, for preferential imaging of NADH and FAD Quadratic discriminant analysis

Sensitivity

Specificity

Sensitivity 94.6

Specificity 85.7

Kumar et al.43 Custom portable autofluorescence device using collimating lens and bream splitter; 405 nm dioxide for excitation Dimensionality reduction using PCA, before Mahalanobis distance classification on first 11 PCs

Sensitivity

Specificity

Accuracy

Sensitivity 98.7

Specificity 100

Accuracy 98.9

Rahman et al.44 Custom portable imaging system composed of modified headlamp system capable of both autofluorescence imaging and reflectance imaging Linear discriminant analysis

Sensitivity

Specificity

AUROC

Sensitivity 92.0

Specificity 84.0

AUROC 0.913

James et al.45 Use of a spectral-domain Optical Coherence Tomography (OCT) system consisting of a 2D scanning long GRID rod probe with a centre wavelength of 930 nm Use of 14 artificial neural networks for feature extraction, followed by support vector machine for classification. Best performance using DenseNet-201 and NASNetMobile in delineating OSCC from others

Sensitivity

Specificity

PPV

NPV

Accuracy

Sensitivity 86.0

Specificity 81.0

PPV 51.0

NPV 96.0

Accuracy 81.9