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. 2022 Aug 13;13:4762. doi: 10.1038/s41467-022-31820-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, c Volcano plots displaying the interactomes of a PARP1-GFP and c GFP-PARP2 after GFP-pull-down from U2OS (FRT) WT cells and analysis by label-free proteomics. b, d Differential interactomes of b PARP1-GFP and d GFP-PARP2 from UV-C-irradiated (20 J/m², 1 h) vs. unirradiated U2OS (FRT) WT cells. a–d The dashed lines indicate a twofold enrichment on the x axis (log₂ of 1) and a significance of 0.05 (−log₁₀ P value of 1.3; two-sided t test) on the y axis. e Enrichment of XPC, ALC1, PARP1, and PARP2 in the co-IPs of PARP1 (a) and PARP2 (b), calculated by intensity-based quantification (IBAQ). Each data point represents a biological replicate (n = 4). f Co-IP of GFP-NLS, PARP1-GFP, and GFP-PARP2 in the presence and absence of UV-C (20 J/m², 1 h). Three independent replicates of each IP experiment were performed obtaining similar results.