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. 2022 Aug 1;12:928373. doi: 10.3389/fonc.2022.928373

Figure 2.

Figure 2

Tumor suppressor gene FBP1 can be activated by miR-24-1 in RCC cells. (A) Left panel: DNA methylation profiling for downregulated tumor suppressor genes in RCC, reporting the depletion of FBP1 in RCC was not caused by hypermethylation in promoter; Right panel: the methylation level of FBP1 promoter between normal and KIRC ones. (B) The expression levels of miR-24-1 in RCC and adjacent normal renal tissues from TCGA database by bioinformatic analysis. (C) The expression levels of miR-24-1 in RCC and adjacent normal renal tissues detected by qPCR. (D) Kaplan–Meier survival analysis showed miR-24-1 is significantly associated with poor overall survival in RCC patients with low expression of miR-24-1 (n=166) compared to high expression of miR-24-1 (n=265). (E) Both miR-24-1 and FBP1 are downregulated in RCC compared to normal ones. Besides, the expression levels of miR-24-1 and FBP1 in RCC tissues exhibit a significantly positive correlation. Correlation coefficient = 0.682. (F, G) The activation of FBP1 by miR-24-1 was assessed by qPCR in 786-O (F) and ACHN (G) cells after transfecting miR-24-1 expression vectors. (H, I) The protein levels of FBP1 in 786-O (H) and ACHN (I) cells were increased after transfecting miR-24-1 confirmed by western blot. Results are shown as mean ± S.D., ****p < 0.0001.