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. 2022 Aug 1;12:928373. doi: 10.3389/fonc.2022.928373

Figure 5.

Figure 5

FBP1 suppresses Warburg effect in RCC cells. (A) Quantification of glucose consumption in miR-24-1-overexpressing 786-O and ACHN cells. (B) Lactate production in 786-O and ACHN cells transfected with miR-24-1 was measured by lactate assay. (C, D) Extracellular acidification rate (ECAR) of 786-O (C) and ACHN (D) cells was detected after miR-24-1 overexpression with glucose starved for two hours and subsequently treated with 2 g/L D-glucose, 1 μM oligomycin, and 100 mM 2-Deoxyglucose (2-DG). (E, F) ECAR represents maximum glycolytic capacity after oligomycin treatment of 786-O (E) and ACHN (F) cells. (G, H) qPCR analysis of glycolytic related genes in vector control or miR-24-1-overexpressing 786-O (G) and ACHN (H) cells. (I) Schematic diagram of FBP1 being activated by miR-24-1 to disturb Warburg effect in RCC cells. The enriched modifications of H3K4me1 and H3K27ac represent active enhancer markers. Results are shown as mean ± S.D. of triplicated experiments, ****p < 0.0001, ***p < 0.001, **p < 0.01.