Figure 6.

MiR-24-1 activates FBP1 via enhancer to suppress tumor growth in vivo. (A) Measuring tumor sizes every 3 days during feeding period. The day when tumors were initially formed marked as day 0. Mice were sacrificed on day 30. (B, C) The weight and size of tumors were calculated. (D) The dissected tumors from sacrificed mice were photographed. (E) qPCR assay was performed to detect the expression levels of miR-24-1 and FBP1 in the tumor tissues among three groups. (F) Representative images of HE staining of tumors. HE staining confirmed that the tumors in each group were indeed RCCs (magnification, 500×). (G) Representative images of immunohistochemistry of tumors. The expression of Ki-67 was obviously less in the tumors from overexpressing miR-24-1 group than those from control group and enhancer-deleted group (top). The expression of FBP1 was increased by overexpressing miR-24-1, but not when enhancer was deleted (middle). The number of TUNEL-positive cells (green fluorescent signal) in the overexpressing miR-24-1 group was significantly increased, indicating an increase of the apoptosis of tumor cells in this group (bottom) (magnification, 500×). Results are shown as mean ± S.D., **p < 0.01, *p < 0.05, ****p < 0.0001, ns means not significant.