FIG. 4.

Inhibition by antibodies to N-terminal CshA of bacterial-cell adhesion to fibronectin. Radioactively labeled cells of E. faecalis OB516 (A) or S. gordonii DL1 (B) were mixed with immune or preimmune antiserum diluted appropriately, and portions (2 × 10⁷ per well) were applied to microtiter plate wells coated with human fibronectin (1 μg per well) or to wells blocked with bovine albumin. The numbers of cells that adhered were measured as described elsewhere (31). Data are presented as percent inhibition of adhesion in the presence of immune serum compared with adhesion in the presence of preimmune serum. The results for CshA antibody inhibition of S. gordonii cells binding to fibronectin are as previously reported (31).