FIG. 6.

Immunogold-labeled E. faecalis OB516(pAMCshA) cells. Cells from the early-stationary phase of growth were incubated with N-terminus-specific CshA antibodies and a gold-conjugated secondary antibody. (A) A newly-divided cell binds gold label that is restricted to one-half of the cell as defined by the nascent septum; (B) sparse deposition of gold particles in the septal region of the cell in the process of cell wall synthesis prior to cell division. Gold particles were located 61.7 ± 12.2 nm from the cell surface. Bars, 200 nm.