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. 2022 Aug 15;40(9):111300. doi: 10.1016/j.celrep.2022.111300

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© 2022 The Author(s)

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Pseudouridine and N1-methylpseudouridine slow down peptidyl transfer by the ribosome

(A) A representative electrophoretic TLC of triplicates showing the translation products of three different initiation complexes—UAC mRNA, ΨAC mRNA, or m1ΨAG mRNA—in the absence and presence of cognate tRNA (Tyr-tRNA^Tyr tRNA) ternary complex.

(B) A representative time course plot of triplicates showing the kinetics of f-Met-Tyr peptide formation in the presence of unmodified (U), pseudouridine (Ψ)-containing, and N1-methylpseudouridine (m1Ψ)-containing A-site UAC codon.

(C and D) Bar graph showing the measured observed rates of peptide-bond formation and reaction endpoints, respectively, in the presence of 1 μM initiation complex and 2.5 μM tRNA^Tyr•EFTu•GTP ternary complex.

Plotted are the average values determined from three independent time courses, with error bars representing the standard deviation around the mean. p values are denoted above the plots.