Figure 6.

The fidelity of reverse transcriptase enzymes is altered in the presence of Ψ

(A) Phosphorimage of a denaturing urea gel used to follow primer extension by M-MLV in the presence of the indicated modified template and dNTP substrates. Shown is a representative gel of duplicates.

(B–D) Bar graphs showing the frequency of variant bases in the codon containing the modified base, as well as the previous codon, as detected by deep sequencing. Bars represent the sum of all variant frequencies, with the proportion for each variant denoted by color. Reference bases are shown below, with the modified base denoted in red. No variants in the two-codon window were detected as statistically significant for the AMV-reverse transcribed m1Ψ RNA.