Figure 4.

YAP1 inhibited ferritinophagy by disrupting the NCOA4−FTH1 interaction. (A) Cells in the indicated groups were transfected with the LC3-GFP plasmid, and the autophagosome in cells presented green fluorescence puncta. Representative confocal images of cells exhibited fluorescent colocalization (yellow puncta) of ferritin (red fluorescence) with LC3-GFP (green puncta). Cells were treated with LPS for 24 (h) (B) Fluorescence quantification of ferritin colocalizing with autophagosomes (LC3-GFP). The colocalization puncta per cell were calculated. (C) Fluorescence quantification of autophagosomes (LC3-GFP). The autophagosome puncta per cell were calculated. (D) Immunoprecipitation analysis of NCOA4 and FTH1 interaction in MLE-12 cells. IgG was used for control. Data are expressed as mean ± SD. n = 3; *p < 0.05, **p < 0.01, ***p < 0.001.