TABLE 1.
Bacterial strains and plasmids
Strain or plasmid | Genotype or relevant phenotype | Source or reference |
---|---|---|
S. typhimurium | ||
14028s | Wild type | ATCCa |
CS019 | 14028s phoN2 zxx::6251 Tn10d-Cm | 34 |
CS401 | CS019 Strr; wild-type invasion/TTSS | S. I. Miller lab |
CJ010 | CS019 with sirA::Tn10d-Tc | 20 |
IB040 | CS019 with prgH::TnphoA, invasion defective | 3 |
DAP3 | IB040 with sirA::Tn10d | 20 |
CJ022 | DAP3 with pCJ20 | 20 |
JLR020 | DAP3 with pWKSHRV2.3 | This work |
JLR002 | DAP3 with pCJ20a | This work |
JLR010 | DAP3 with pCJ20b | This work |
JLR016 | DAP3 with pCJ20c | This work |
JLR018 | DAP3 with pCJ20d | This work |
CJ023 | DAP3 with pCJ22 | 20 |
HRB002 | DAP3 with pCJ22a | This work |
HRB003 | DAP3 with pCJ22b | This work |
HRB004 | DAP3 with pCJ22c | This work |
HRB005 | DAP3 with pCJ22d | This work |
HRB090 | ΔsirB 1.2-kb in-frame deletion in CS401 | This work |
CS015 | phoP102::Tn10d-Cm PhoP− | 35 |
CS022 | pho-24 PhoPc, PhoP-repressed genes constitutively repressed | 35 |
VV341 | hilA::kan | 2 |
JLR158 | CS401 with hilA::kan from VV341 | This work |
JLR028 | sirC::luc in CS401, measures sirC expression in the presence of SirC | This work |
JLR077 | CS015 with sirC::luc from JLR028 | This work |
JLR076 | CS022 with sirC::luc from JLR028 | This work |
JLR027 | JLR028 with hilA::kan from VV341 | This work |
JLR040 | CJ010 with sirC::luc from JLR028 | This work |
HRB094 | HRB090 with sirC::luc from JLR028 | This work |
JLR053 | ΔsirC 748-bp in-frame deletion in CS401 | This work |
CL87 | iagB::lacZY measures hilA expression in the presence of HilA, wild-type invasiveness | Gift of C. Lee |
JLR129 | CS401 with iagB::lacZY from CL87 | This work |
JLR130 | JLR053 with iagB::lacZY from CL87 | This work |
JLR151 | JLR129 with sirA::Tn10d from CJ010 | This work |
JLR152 | JLR130 with sirA::Tn10d from CJ010 | This work |
EE638 | sspC::Tn5-lacZY | 1 |
EE637 | invF::Tn5-lacZY | 1 |
EE656 | prgH::Tn5-lacZY | 2 |
JLR138 | CS401 with sspC::Tn5-lacZY from EE638 | This work |
JLR135 | CS401 with invF::Tn5-lacZY from EE637 | This work |
JLR136 | CS401 with prgH::Tn5-lacZY from EE656 | This work |
JLR141 | JLR138 with hilA::kan from VV341 | This work |
JLR149 | JLR135 with hilA::kan from VV341 | This work |
JLR140 | JLR136 with hilA::kan from VV341 | This work |
JLR147 | JLR141 with pCJ20 | This work |
JLR150 | JLR149 with pCJ20 | This work |
JLR145 | JLR140 with pCJ20 | This work |
JLR153 | JLR141 with pCJ13d | This work |
JLR155 | JLR149 with pCJ13d | This work |
JLR156 | JLR140 with pCJ13d | This work |
Plasmids | ||
pWSK29 | Ampr, low-copy-number cloning vector | 9 |
pWKS30 | Ampr, low-copy-number cloning vector | 9 |
pWKSHRV2.3 | Ampr, 2.3-kb HindIII-EcoRV fragment (contains all of orgA) | Gift of C. Lee and V. Bajaj |
pCJ20 | Ampr, contains 4.4 kb of sirC region | 20 |
pCJ20a-d | Ampr (see Fig. 1) | This work |
pCJ22 | Ampr, contains 4.6 kb of sirB region | 20 |
pCJ22a-d | Ampr (see Fig. 1) | This work |
pCJ13d | Ampr, contains sirA expressed from its own promoter | 20 |
pGPL01 | Ampr, luciferase suicide vector | 17 |
psirC::luc | Ampr, 1-kb HindIII-NruI fragment from pCJ20 in pGPL01 | This work |
pKAS32 | Ampr Strs, pGP704-based suicide vector | 39 |
pΔsirC | Ampr Strs, used to create deletion of 748 bp of sirC | This work |
pΔsirB | Ampr Strs, used to create deletion of 1.2 kb of sirB ORF1 and ORF2 | This work |
ATCC, American Type Culture Collection.