TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or relevant phenotype | Source or reference |
|---|---|---|
| S. typhimurium | ||
| 14028s | Wild type | ATCCa |
| CS019 | 14028s phoN2 zxx::6251 Tn10d-Cm | 34 |
| CS401 | CS019 Strr; wild-type invasion/TTSS | S. I. Miller lab |
| CJ010 | CS019 with sirA::Tn10d-Tc | 20 |
| IB040 | CS019 with prgH::TnphoA, invasion defective | 3 |
| DAP3 | IB040 with sirA::Tn10d | 20 |
| CJ022 | DAP3 with pCJ20 | 20 |
| JLR020 | DAP3 with pWKSHRV2.3 | This work |
| JLR002 | DAP3 with pCJ20a | This work |
| JLR010 | DAP3 with pCJ20b | This work |
| JLR016 | DAP3 with pCJ20c | This work |
| JLR018 | DAP3 with pCJ20d | This work |
| CJ023 | DAP3 with pCJ22 | 20 |
| HRB002 | DAP3 with pCJ22a | This work |
| HRB003 | DAP3 with pCJ22b | This work |
| HRB004 | DAP3 with pCJ22c | This work |
| HRB005 | DAP3 with pCJ22d | This work |
| HRB090 | ΔsirB 1.2-kb in-frame deletion in CS401 | This work |
| CS015 | phoP102::Tn10d-Cm PhoP− | 35 |
| CS022 | pho-24 PhoPc, PhoP-repressed genes constitutively repressed | 35 |
| VV341 | hilA::kan | 2 |
| JLR158 | CS401 with hilA::kan from VV341 | This work |
| JLR028 | sirC::luc in CS401, measures sirC expression in the presence of SirC | This work |
| JLR077 | CS015 with sirC::luc from JLR028 | This work |
| JLR076 | CS022 with sirC::luc from JLR028 | This work |
| JLR027 | JLR028 with hilA::kan from VV341 | This work |
| JLR040 | CJ010 with sirC::luc from JLR028 | This work |
| HRB094 | HRB090 with sirC::luc from JLR028 | This work |
| JLR053 | ΔsirC 748-bp in-frame deletion in CS401 | This work |
| CL87 | iagB::lacZY measures hilA expression in the presence of HilA, wild-type invasiveness | Gift of C. Lee |
| JLR129 | CS401 with iagB::lacZY from CL87 | This work |
| JLR130 | JLR053 with iagB::lacZY from CL87 | This work |
| JLR151 | JLR129 with sirA::Tn10d from CJ010 | This work |
| JLR152 | JLR130 with sirA::Tn10d from CJ010 | This work |
| EE638 | sspC::Tn5-lacZY | 1 |
| EE637 | invF::Tn5-lacZY | 1 |
| EE656 | prgH::Tn5-lacZY | 2 |
| JLR138 | CS401 with sspC::Tn5-lacZY from EE638 | This work |
| JLR135 | CS401 with invF::Tn5-lacZY from EE637 | This work |
| JLR136 | CS401 with prgH::Tn5-lacZY from EE656 | This work |
| JLR141 | JLR138 with hilA::kan from VV341 | This work |
| JLR149 | JLR135 with hilA::kan from VV341 | This work |
| JLR140 | JLR136 with hilA::kan from VV341 | This work |
| JLR147 | JLR141 with pCJ20 | This work |
| JLR150 | JLR149 with pCJ20 | This work |
| JLR145 | JLR140 with pCJ20 | This work |
| JLR153 | JLR141 with pCJ13d | This work |
| JLR155 | JLR149 with pCJ13d | This work |
| JLR156 | JLR140 with pCJ13d | This work |
| Plasmids | ||
| pWSK29 | Ampr, low-copy-number cloning vector | 9 |
| pWKS30 | Ampr, low-copy-number cloning vector | 9 |
| pWKSHRV2.3 | Ampr, 2.3-kb HindIII-EcoRV fragment (contains all of orgA) | Gift of C. Lee and V. Bajaj |
| pCJ20 | Ampr, contains 4.4 kb of sirC region | 20 |
| pCJ20a-d | Ampr (see Fig. 1) | This work |
| pCJ22 | Ampr, contains 4.6 kb of sirB region | 20 |
| pCJ22a-d | Ampr (see Fig. 1) | This work |
| pCJ13d | Ampr, contains sirA expressed from its own promoter | 20 |
| pGPL01 | Ampr, luciferase suicide vector | 17 |
| psirC::luc | Ampr, 1-kb HindIII-NruI fragment from pCJ20 in pGPL01 | This work |
| pKAS32 | Ampr Strs, pGP704-based suicide vector | 39 |
| pΔsirC | Ampr Strs, used to create deletion of 748 bp of sirC | This work |
| pΔsirB | Ampr Strs, used to create deletion of 1.2 kb of sirB ORF1 and ORF2 | This work |
a
ATCC, American Type Culture Collection.