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. 2022 Aug 1;9:938989. doi: 10.3389/fnut.2022.938989

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Copyright © 2022 Azam and Khan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A,B) Biofilm reduction quantification was done through CV assay in csgD-KD cells. (A) TKA showed 61.47% and TKB 48.09% biofilm reduction, respectively, compared to the control cells. (B) The EcN csgD-KD mutants TEA and TEB showed 75.6% and 64.7% biofilm reduction. (C,D) XTT reduction assay for cell viability in csgD csgD-KD cells. (C) Cell viability of csgD-KD cells of K12 (TKA and TKB) (D) Cell viability of csgD-KD cells of EcN (TEA and TEB). The data shown here represent an average value of triplicate experiments ± S (*P < 0.05, t-test, two-sided), (**P < 0.005, t-test, two-sided).