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. 2022 Aug 1;9:938989. doi: 10.3389/fnut.2022.938989

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Copyright © 2022 Azam and Khan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A,B) The spectra showing Congo red fluorescence assay of csgD-KD cells. The control cell showed a higher fluorescence intensity as compared to the csgD-KD cells (A) Congo red fluorescence assay of csgD-KD cells of K12 (TKA and TKB) (B) Congo red fluorescence assay of csgD-KD cells of EcN (TEA and TEB). (C,D) The spectra are showing ThT (thioflavin) fluorescence assay in csgD-KD cells. The decline in fluorescence spectra intensity suggests lower curli amyloid production in csgD-KD cells, (C) ThT fluorescence assay of TKA and TKB, (D) ThT fluorescence assay of TEA and TEB.