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. Author manuscript; available in PMC: 2022 Aug 15.
Published in final edited form as: Cell Rep. 2022 May 3;39(5):110755. doi: 10.1016/j.celrep.2022.110755

Figure 1. Noncanonical inflammasome activation by LPS in vivo is CD14-dependent.

Figure 1.

(A, C, and D) Plasma IL-18 (A and C) and IL-1β (D) in IFN-γ (1 μg; 3 h)-primed mice 6 h post-LPS (20 mg/kg) injection.

(B and E) Full-length and cleaved GSDMD (GSDMD-FL and GSDMD-N, respectively), caspase-11, and NLRP3 in spleen homogenates from indicated mice treated as above.

(F and G) Survival (F; n = 10 for each genotype) and body temperature (G; n = 10 for each genotype) of indicated mice primed with IFN-γ (0.25 μg; 3 h) and injected with LPS (10 mg/kg).

Data from two experiments (A, F, and G) or one experiment representative of two (B–E) are shown. Each circle represents a mouse, and horizontal lines represent mean (A–E). Data are presented as the mean ± SEM (G). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns: non-significant; one-way ANOVA/Dunnett’s test (A and B), unpaired two-tailed t test (C–E), Mantel-Cox test (F), or two-way ANOVA/Sidak’s test (G).

See also Figure S1.