KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-human/mouse NLRP3 (Clone D4D8T) | Cell Signaling Technology | Cat# 15101; RRID:AB_2722591 |
| Rabbit monoclonal anti-mouse GSDMD (Clone EPR 19828) | Abcam | Cat# Ab209845; RRID:AB_2783550 |
| Rat monoclonal anti-mouse Caspase 11 (Clone 17D9) | Cell Signaling Technology | Cat# 14340S; RRID:AB_2728693 |
| Anti-Rat IgG HRP | Jackson ImmunoResearch Labs | Cat# 712-035-150; RRID:AB_2340638 |
| Anti-Rabbit IgG HRP | Jackson ImmunoResearch Labs | Cat# 711-035-152; RRID:AB_10015282 |
| Rat monoclonal anti-IL-18 antibody (Clone 74; ELISA capture antibody) | MBL International | Cat# D047-3; RRID:AB_592016 |
| Rat monoclonal anti-IL-18 antibody Biotin (Clone 93-10C; ELISA detection antibody) | MBL International | Cat# D048-6; RRID:AB_592012 |
| APC-anti-mouse/human CD11b antibody (Clone M1/70) | BioLegend | Cat# 101212; RRID:AB_312795 |
| FITC-anti-mouse/human CD11b antibody (Clone M1/70) | BioLegend | Cat# 101206; RRID:AB_312789 |
| Monoclonal mouse anti-fluorescein (FITC) antibody (Clone 1F8.1E4) | Biotium | Cat# 20210 |
| APC-anti-mouse CD45.2 antibody (Clone 104) | Invitrogen | Cat# 17-0454-82 |
| Rabbit monoclonal anti-human/mouse/rat EEA1 (Clone 45B10) | Cell Signaling Technology | Cat# 3288S; RRID:AB_2096811 |
| Rat monoclonal anti-mouse CD107a (Lamp1) (Clone eBio1D4B) | eBioscience | Cat# 14-1071-85; RRID:AB_657533 |
| APC-anti-mouse CD14 (Clone Sa2-8) | Invitrogen | Cat# 17-0141-81; RRID:AB_469352 |
| Rabbit polyclonal anti-human/mouse/rat Galectin-3 (Clone D4I2R) | Cell Signaling Technology | Cat# 12733; RRID:AB_2798009 |
| Mouse monoclonal Rab5A (Clone E6N8S) | Cell Signaling Technology | Cat# 46449S; RRID:AB_2799303 |
| CF405M-Goat anti-rat IgG (H+L) | Biotium | Cat# 20374 |
| CF405M-Goat anti-rabbit IgG (H+L) | Biotium | Cat# 20373 |
| CF647-Goat anti-rabbit IgG (H+L) | Biotium | Cat# 20282; RRID:AB_10853478 |
| Mouse monoclonal anti-FITC gold conjugate | Electron Microscopy Sciences | Cat# 25581 |
|
Chemicals, peptides, and recombinant proteins | ||
| LPS E. coli O111:B4 | Sigma | Cat# L3024-25MG |
| FITC-conjugated LPS E. coli O111:B4 | Sigma | Cat# F3665-10MG |
| Recombinant mouse IFN-γ | R&D Systems | Cat# 485-MI-100 |
| Collagenase | Sigma Aldrich | Cat# C5138-5G |
| DNase I | Sigma Aldrich | Cat# DN25-1G |
| Recombinant mouse IL-18 (ELISA standard) | MBL | Cat# B002-5 |
| Pam3CSK4 | InvivoGen | Cat# tlrI-pms |
| Recombinant HMGB1 | HMGBiotech | Cat# HM-114 |
| RAGE inhibitor FPS-ZM1 | Cayman | Cat# 11909 |
| RAGE Antagonist Peptide (RAP) | Millipore | Cat# 553031 |
|
Critical commercial assays | ||
| Mouse IL-1β ELISA kit | Thermo Fisher Scientific | Cat# 50-171-85 |
| Mouse TNF ELISA kit | Thermo Fisher Scientific | Cat# 88-7324 |
| Mouse IL-1α ELISA kit | Thermo Fisher Scientific | Cat# 88-5019 |
| Mouse Galectin-1 ELISA kit | R&D Systems | Cat# DY1245 |
| Pierce™ BCA Protein Assay kit | Thermo Fisher Scientific | Cat# 23227 |
| Halt™ Protease Inhibitor Cocktail (100X) | Thermo Fisher Scientific | Cat# 1861279 |
| NuPAGE LDS sample buffer (4X) | Invitrogen | Cat# NP0007 |
| Trans-Blot Turbo Transfer System | Bio-Rad | Cat# 1704271 |
| Clarity ECL HRP Substrate | Bio-Rad | Cat# 170-5060S |
| Mouse Coagulation Factor III/TF ELISA kit | R&D systems | Cat# DY3178-05 |
| Pacific Hemostasis Thromboplastin-D | Thermo Scientific | Cat# 100356TS |
| ACK Lysis buffer | Gibco | Cat# A1049201 |
| Chromo-LAL | Associates of Cape Cod | Cat# C0031-5 |
| Glucashield® (1→3)-ß-D-Glucan Inhibiting Buffer | Associates of Cape Cod | Cat# GB051-5 |
| HEK-Blue Detection | Invivogen | Cat# hb-det2 |
| HEK-Blue Selection | Invivogen | Cat# hb-sel |
| Lipofectamine 2000 | Invitrogen | Cat# 11668027 |
| GoldEnhance EM kit | Nanoprobes | Cat# 2113-8ML |
|
Experimental Models: Cell lines | ||
| HEK-Blue™ hTLR4 | InvivoGen | Cat# hkb-htlr4 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | RRID:IMSR_JAX:000664 |
| Mouse: Casp11−/− | Kayagaki et al., Nature 2011 Genentech | N/A |
| Mouse: Tlr4−/− | The Jackson Laboratory | RRID:IMSR_JAX:007227 |
| Mouse: Cd14−/− | The Jackson Laboratory | RRID:IMSR_JAX:003726 |
| Mouse: Tlr4−/−Cd14−/− | Generated in this study | N/A |
| Mouse: Tlr4−/−Casp11−/− | Generated in this study | N/A |
|
Oligonucleotides | ||
| Casp11−/− genotyping Primer 1: CCCTGGAAAAATCGATGACT | Kayagaki et al., Nature 2011 & Integrated DNA Technologies | Mouse listed above |
| Casp11−/− genotyping Primer 2: TGAAATGCATGTACTGAGAGCAAGG | Kayagaki et al., Nature 2011 & Integrated DNA Technologies | Mouse listed above |
| Casp11−/− genotyping Primer 3: CAATTGACTTGGGGATTCTGG | Kayagaki et al., Nature 2011 & Integrated DNA Technologies | Mouse listed above |
| Tlr4−/− genotyping Primer 1 oIMR8365: GCAAGTTTCTATATGCATTCTC | The Jackson Laboratory & Integrated DNA Technologies | Mouse listed above |
| Tlr4−/− genotyping Primer 2 oIMR8366: CCTCCATTTCCAATAGGTAG | The Jackson Laboratory & Integrated DNA Technologies | Mouse listed above |
| Tlr4−/− genotyping Primer 3 oIMR8367: TATGCATGATCAACACCACAG | The Jackson Laboratory & Integrated DNA Technologies | Mouse listed above |
| Tlr4−/− genotyping Primer 4 oIMR8368: TTTCCATTGCTGCCCTATAG | The Jackson Laboratory & Integrated DNA Technologies | Mouse listed above |
| Cd14−/− genotyping Primer 1 oIMR0662: CCGCTTCCATTGCTCAGCGG | The Jackson Laboratory & Integrated DNA Technologies | Mouse listed above |
| Cd14−/− genotyping Primer 2 oIMR1314: CCAAGTTTTAGCGCTGCGTAAC | The Jackson Laboratory & Integrated DNA Technologies | Mouse listed above |
| Cd14−/− genotyping Primer 3 oIMR1315: GCCAGCCAAGGATACATAGCC | The Jackson Laboratory & Integrated DNA Technologies | Mouse listed above |
|
Software and Algorithms | ||
| GraphPad Prism 9.3 | GraphPad Software | N/A |
| Fiji/ImageJ (version 10.2) | https://imagej.net/Fiji | N/A |