Figure 6.

Fidgetin knockout specifically induced compensative upregulation of fidgetin-like 1.

A and B: Semi-quantitative (A) and quantitative real-time (B) RT-PCR were performed to compare the mRNA levels of each microtubule-severing protein (i.e., fidgetin [Fign], spastin [Spast], katanin-like 1[Katnal1], katanin-like 2 [Katnal2], katanin [Katna1], fidgetin-like 2 [Fignl2], and fidgetin-like 1 [Fignl1]) between wild type and fidgetin knockout ovaries. N=3 (A) or 5 (B). C: DNA sequence alignment was performed to show the similarity between 176–342 bp of the 5′ UTR of Fign mRNA and 60–208 bp of the 5′ UTR of Fignl1 mRNA. Gapdh was used as the internal reference gene. Student's t-test was used for statistics between two groups in A and B. ^*P<0.05. AU: arbitrary unit.