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. Author manuscript; available in PMC: 2022 Aug 15.
Published in final edited form as: Insect Biochem Mol Biol. 2021 Aug 3;137:103625. doi: 10.1016/j.ibmb.2021.103625

Fig. 4.

Fig. 4.

Charge reversal of two negatively-charged residues in IIIS2 increase the effect of Lqh-dprIT3. A-E, Conductance-voltage relations in WT BgNav1–1a channels (A) or the four channel mutants substituted in domain III (B-E) in the absence (●) or presence (○) of 300 nMLqh-dprIT3. To measure the toxin effect, a 20 Hz train (50) of 5 ms depolarizing prepulses to 50 mV from a holding potential of −120 mV was followed by a series of 20 ms depolarizing test pulses between −80 and −65 mV. F, Percentage of modification of the WT and four mutant channels elicited by Lqh-dprIT3. * denotes a significant difference compared to WT BgNav1–1a channels, as determined by one-way ANOVA with Scheffe’s post hoc analysis (p˂ 0.05).