Table 2.
Voltage dependence of activation of BgNav1–1a and of the four negatively-charged channel mutants before and after the application of 300 nM Lqh-dprIT3.
| Toxin-free |
Lqh-dprIT3 (300 nM) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| V0.5k | V0.5 | k 1 | A1 (%) | V0.5 | k 2 | A2 (%) | ||||
|
| ||||||||||
| BgNav1–1a | −31.3 ± 2.2 | 4.9 ± 0.3 | −32.0 ± 1.9 | 3.9 ± 0.3 | 72 ± 5 | −68.0 ± 4.6 | 8.5 ± 1.0 | 28 ± 1 | ||
| IIIS1-E1K | −31.2 ± 1.1 | 5.2 ± 0.2 | −33.7 ± 1.2 | 4.4 ± 0.2 | 77 ± 4 | −70.7 ± 2.6 | 9.2 ± 0.5 | 23 ± 4 | ||
| IIIS1-E2K | −31.2 ± 0.5 | 5.7 ± 0.2 | −34.2 ± 1.0 | 4.7 ± 0.2 | 69 ± 7 | −75.0 ± 2.1 | 11.0 ± 0.8 | 31 ± 7 | ||
| IIIS2-D1K | −25.9 ± 0.8 | 7.6 ± 0.2 | −55.2 ± 5.8 | 4.3 ± 0.8 | 25 ± 6 | −75.0 ± 3.5 | 5.4 ± 0.5 | 75 ± 6a | ||
| IIIS2-E2K | −29.4 ± 1.2 | 7.5 ± 0.3 | −30.7 ± 1.8 | 4.8 ± 0.6 | 33 ± 6 | −69.9 ± 1.9 | 7.7 ± 0.9 | 67 ± 6a | ||
Each value represents the mean ± SEM for at least eight oocytes.
a
Significant differences from other mutants were determined by one-way ANOVA analysis of variance (p ˂ 0.05).