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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: J Control Release. 2022 May 23;347:521–532. doi: 10.1016/j.jconrel.2022.05.020

Fig. 3.

Fig. 3.

Screen of LNP library for luciferase mRNA delivery and viability in Jurkats to identify top formulations. Jurkat cells were treated with LNP formulations at 60 ng mRNA / 60,000 cells for 24 h. Luciferase expression was normalized to cells treated with the standard LNP formulation (S2), and background luminescence was subtracted. Percent viability of cells treated with LNPs was determined by normalization to untreated cells. Legend denotes percent substitution of each hydroxycholesterol substitute into the S2 formulation. n = 3 biological replicates. Error bars denote standard deviation. An ANOVA was used to determine if treatment group means differed significantly. **: p < 0.01 in Tukey's honest significance test between LNP candidate and S2.