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Optimized HPTLC conditions for the analysis of baicalin
| HPTLC instrument | CAMAG Linomat 5 |
|---|---|
| Stationary phase | Silica gel 60 G F254 |
| Mobile phase | Acetone‒ethyl acetate‒water‒formic acid (2:10:0.5:0.5, V/V) |
| Observed RF values | Baicalin (0.49) |
| Band width | 6 mm |
| Saturation time | 30 min |
| Solvent front distance | 85 mm |
| Plate activation time | 15 min |
| Detection lamp | Deuterium |
| Detection wavelength | 318 nm |
| Scanning rate | 20 mm/s |
