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. 2022 Jul 28;13:890298. doi: 10.3389/fimmu.2022.890298

Figure 2.

Figure 2

Activation and proliferation of MAIT cells by fungal extracts. PBMCs were incubated for 24 h with SC and CA extracts. At the end of the incubation, the cells were labeled with CD3, CD4, CD8, Vα7.2, CD161, and CD69 and analyzed on a flow cytometer. (A) Representative plot of CD69 expression measured on the different cell populations indicated. (B) Cumulative date of activation of CD69 (HD n = 6; NEDA MS n = 8). Bars represent mean with SD. (C) MAIT cell proliferation following incubation with CA and SC. PBMCs from pwMS and HD were stained with CFSE and incubated for 5 days in the presence of the fungal extracts. On the fifth day, the cells were collected and stained with CD3, CD8, CD161, and Vα7.2, and the percentage of proliferating cells was measured based on the dilution of CFSE (n = 4). Bars represent mean with SD. Statistical significance was assessed by unpaired (between groups) or paired (between experimental conditions) t-test. *p < 0.05.