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Biochemical and cellular activity of MKK7 inhibitors. (A, B) in vitro kinase activity assays for compounds (A) 1, 1b, and 1k and (B) 2 and 2a (see Figure S12 for full inhibition curve of 2). (C–F) Quantification of dose–response c-Jun phosphorylation inhibition by a western blot assay: (C) 1, (D) 2, (E) analogues 1a, 1b, 1d, 1e, and 1k, and (F) analogues 2a, 2b, and 2c. See Figure S10 for the raw WB analysis.
