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(A–D) Confocal images of fixed Cos7 cells stained with secondary antibodies against microtubules labeled with the indicated DAE before (top) and after (bottom) CB7 addition. The markers are switched on before the recording of each pixel, with a short pulse of 355 nm light. (E) Average signal intensity of selected single filaments before (green) and after (orange) CB7 addition. (F) Normalized intensity distribution of selected single filaments (N = 50). Scale bar in A–D: 10 μm.
