Table 3.

Comparison of Se400 sequence specificity with other introduced genes for detecting S. epidermidis in species level

Target	Length	Similarity to non-S. epidermidis genomes with >35% query cover and >60% identity
Se400	400 bp	No similarity to any non-S. epidermidis microbes
gseA (2004)	1214 bp	3 strains of S. saccharolyticus with >68% query cover and >75% identity 5 strains of S. caprae with >58% query cover and >69% identity 11 strains of S. warneri with 55% query cover and 67% identity >1300 strain of S. aureus with 50% query cover and 67–69% identity 6 strains of S. pasteur with 46–51% query cover and 67–69% identity
serp0107 (2006)	882 bp	3 strains of S. saccharolyticus with 98% query cover and 69% identity 7 strains of S. capitis with 95% query cover and 68% identity 5 strains of S. caprae with 66% query cover and 70% identity 3 strains of S. haemolyticus with 42% query cover and >68% identity Other microorganisms: S. warneri S. hominis, S. argenteus
sesC (2016)	2031 bp	3 strains of S. saccharolyticus with 83–100% query cover and >69% identity 7 strains of S. capitis with 42% query cover and >67% identity 5 strains of S. caprae with 52% query cover and 66% identity
ecpB (2019)	318 bp	3 strains of S. saccharolyticus with 100% query cover and >77% identity 19 strain of S. aureus with 65% query cover and 67% identity

The specificity of the genes was tested bioinformatically using Blastn software.