Table 3. Introducing a Methyl Substituent to the Indole Ring.
| compd | R1 | R2 | R3 | R4 | R5 | rh-renin IC50 (FRET, nM)a | hPRA IC50 (nM)b | MW | ClogP | BEI |
|---|---|---|---|---|---|---|---|---|---|---|
| 15 | H | H | H | H | H | 13 | 84 | 371.5 | 2.30 | 21.2 |
| 16 | Me | H | H | H | H | >10000 | 385.5 | 2.75 | ||
| 17 | H | Me | H | H | H | 5.8 | 54 | 385.5 | 2.80 | 21.3 |
| 18 | H | H | Me | H | H | 274 | 385.5 | 2.80 | 17.0 | |
| 19 | H | H | H | Me | H | 19 | 74 | 385.5 | 2.80 | 20.0 |
| 20 | H | H | H | H | Me | 9.3 | 103 | 385.5 | 2.80 | 20.8 |
IC50 values are shown as the mean of duplicate measurements and calculated from the concentration–inhibition curve using Origin software (version 5.0, Microcal Software, Inc.).
IC50 values are shown as the mean of duplicate measurements and calculated from the concentration–inhibition curve using SAS software (version 9.1.3, SAS Institute Inc.).
