Figure 4.

Representative Western blot of endogenous GLI1 in NIH3T3 cells stimulated with the Smoothened agonist SAG (100 nM) and treated with vehicle (DMSO), our putative GLI1 inhibitors (1 μM), and GANT61 (5 μM) for 48 h. HSP90 was used as loading control. See Table 1 for details.