Table 1. Chemical Structure, Antiproliferative Activity, and Effect on GLI1 Properties of the Quinoline and Oxazino-Quinoline Derivatives.

^aCompounds also labeled with BAS and AKos notations were purchased from Asinex and AKos vendors, respectively.

^bExpressed as IC₅₀ values (micromolar concentrations) calculated using GraphPad Prism V6 from triplicate experiments in melanoma (A375) and medulloblastoma (DAOY) cells. ND: not determined.

^cThe GLI1 protein level was determined by Western blotting (see Figure 4 for details) in murine NIH3T3 cells treated with SAG (100 nM), each compound (1 μM), and GANT61 (5 μM) for 48 h. NA: not affected; weakly reduced: +; reduced: ++; strongly reduced: +++.

^dActivity data taken from ref (18).