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Genes identified in the 2,4-DAPG biosynthetic locus are indicated by black horizontal arrows. The vertical arrow indicates the site of Tn5 insertion in Q2-87::Tn5-1. Key plasmids used in assigning functions to the phl genes are shown. Construction of these plasmids is described in Materials and Methods. Restriction sites: A, AccI, B, BamHI; E, EcoRV; Eg, EagI; P, PstI; R, EcoRI; S, SalI; Sp, SphI; St, StuI.
